![]() The cDNAs were isolated by using degenerate oligonucleotides based on the sequence of the central region of somatostatin to screen a frog brain cDNA library. The present study reports the characterization of the cDNA clones that encode two distinct somatostatin precursors in the brain of the frog Rana ridibunda. N2 - In tetrapods, only one gene encoding a somatostatin precursor has been identified so far. T2 - Characterization of the cDNAs, distribution of the mRNAs, and receptor-binding affinities of the peptides T1 - Occurrence of two somatostatin variants in the frog brain These data also suggest that distinct genes encoding somatostatin variants may be expressed in the brain of other tetrapods.", The present study indicates that the two somatostatin variants could exert different functions in the frog brain and pituitary. The somatostatin variant SS2 was significantly more potent (4-6 fold) than somatostatin itself in displacing somatostatin-14 from its specific binding sites. ![]() In situ hybridization studies indicate differential expression of the PSS1 and PSS2 genes in the septum, the lateral part of the pallium, the amygdaloid complex, the posterior nuclei of the thalamus, the ventral hypothalamic nucleus, the torus semicircularis and the optic tectum. The other cDNA encodes a 103-amino acid protein (prepro-somatostatin-14 PSS2) that contains the sequence of the somatostatin analog (peptide SS2) at its C terminus, but does not exhibit appreciable sequence similarity with PSS1 in the remaining region. One of the cDNAs encodes a 115-amino acid protein (prepro-somatostatin-14 PSS1) that exhibits a high degree of structural similarity with the mammalian somatostatin precursor. These data also suggest that distinct genes encoding somatostatin variants may be expressed in the brain of other tetrapods.Ībstract = "In tetrapods, only one gene encoding a somatostatin precursor has been identified so far. ![]() ![]() Expression Biased expression in Brain (RPKM 9.9) and Testes (RPKM 2.In tetrapods, only one gene encoding a somatostatin precursor has been identified so far. Orthologous to human HCRT (hypocretin neuropeptide precursor). Human ortholog(s) of this gene implicated in narcolepsy. Involved in several processes, including G protein-coupled receptor signaling pathway regulation of metal ion transport and regulation of transmission of nerve impulse. Full Name hypocretin neuropeptide precursor provided by RGD Primary source RGD:2786 See related Ensembl:ENSRNOG00000018892 AllianceGenome:RGD:2786 Gene type protein coding RefSeq status VALIDATED Organism Rattus norvegicus Lineage Eukaryota Metazoa Chordata Craniata Vertebrata Euteleostomi Mammalia Eutheria Euarchontoglires Glires Rodentia Myomorpha Muroidea Muridae Murinae Rattus Also known as orexin-A Summary Enables neuropeptide hormone activity type 1 hypocretin receptor binding activity and type 2 hypocretin receptor binding activity.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |